Three dimensional subsurface elemental identification of minerals using confocal micro-X-ray fluorescence and micro-X-ray computed tomography

Subsurface Particle Analysis using X-ray Computed Tomography and Confocal X- ray Fluorescence

X-ray micro-computed tomography

Micro-computed tomography (μCT) has been successfully used to study the cardiovascular system of mouse embryos in situ. With the use of barium as a suitablecontrast agent, blood vessels have beenimaged and analysed quantitatively such asblood volume and vessel sizes on embryosof ages 14.5 to 16.5 days old. The advantageof using this imaging modality is that it hasprovide...

Quantification of eggshell microstructure using X-ray micro computed tomography

1. X-ray microcomputed tomography can be used to produce rapid, fully analysable, three-dimensional images of biological and other materials without the need for complex or tedious sample preparation and sectioning. We describe the use of this technique to visualise and analyse the microstructure of fragments of shell taken from three regions of chicken eggs (sharp pole, blunt pole and equatori...

Three-dimensional X-ray micro-velocimetry

A direct measurement of three-dimensional X-ray velocimetry with micrometer spatial resolution is presented. The key to this development is the use of a Laue crystal as an X-ray beam splitter and mirror. Three-dimensional flow velocities in a 0.4 mm-diameter tubing were recorded, with <5 µm spatial resolution and speeds of 0.7 mm s(-1). This development paves the way for three-dimensional veloc...

Three-dimensional visualisation of soft biological structures by X-ray computed micro-tomography.

Whereas the two-dimensional (2D) visualisation of biological samples is routine, three-dimensional (3D) imaging remains a time-consuming and relatively specialised pursuit. Current commonly adopted techniques for characterising the 3D structure of non-calcified tissues and biomaterials include optical and electron microscopy of serial sections and sectioned block faces, and the visualisation of...